Annals of Hematology

, Volume 66, Issue 1, pp 27–31

Effects of chemotherapeutic and immunosuppressive drugs on the production of erythropoietin in human hepatoma cultures

  • M. Wolff
  • W. Jelkmann
Original Article

DOI: 10.1007/BF01737686

Cite this article as:
Wolff, M. & Jelkmann, W. Ann Hematol (1993) 66: 27. doi:10.1007/BF01737686


The effects of agents used in combination chemotherapy were studied on erythropoietin synthesis in cultures of the human hepatoma cell line, HepG2. Erythropoietin was measured by radioimmunoassay in the culture media after 24-h treatment periods. The RNA synthesis-inhibiting drugs daunorubicin, cyclophosphamide, ifosfamide, andcis-diamine-dichloroplatinum produced a dose-dependent decrease of the production of erythropoietin. Inhibition was also induced by the tubulin-binding agents vincristine and colchicine and the immunosuppressive agents azathioprine and cyclosporin A. The DNA synthesis-inhibiting drugs methotrexate and cytosine arabinoside, the antimycotics 5-fluorocytosine and amphotericin B, and glucocorticoids did not inhibit. Viability studies showed that the inhibition of erythropoietin production was partly correlated with cytotoxicity. These data could be relevant with respect to recombinant erythropoietin replacement therapy in tumor-associated anemia.

Key words

Erythropoietin Chemotherapy Malignant diseases Anemia 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Wolff
    • 1
  • W. Jelkmann
    • 1
  1. 1.Department of PhysiologyUniversity of BonnBonn 1Federal Republic of Germany

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