Molecular and Cellular Biochemistry

, Volume 6, Issue 2, pp 93–100

Isocitrate dehydrogenases and oxoglutarate dehydrogenase activities of baker's yeast grown in a variety of hypoxic conditions

  • Alberto Machado
  • Ignacio Nuñez de Castro
  • Federico Mayor
General and Review Articles a. general articles

DOI: 10.1007/BF01732003

Cite this article as:
Machado, A., Nuñez de Castro, I. & Mayor, F. Mol Cell Biochem (1975) 6: 93. doi:10.1007/BF01732003

Summary

The activities of isocitrate dehydrogenase (NAD), isocitrate dehydrogenase (NADP) and oxoglutarate dehydrogenase have been investigated inSaccharomyces cerevisiae grown in a variety of aerobic and hypoxic conditions, the latter including oxygen deprivation, high glucose concentration, addition of inhibitors of mitochondrial protein synthesis, respiratory inhibition by azide, and impaired respiration mutants.

All hypoxic conditions led to a marked decrease of oxoglutarate dehydrogenase and significant decreases of the two isocitrate dehydrogenases. According to its kinetic properties, the NAD-isocitrate dehydrogenase will not be operative in hypoxia “in vivo”. From these and other related facts it is concluded that hypoxic conditions in yeast generally lead to a splitting of the tricarboxylic acid cycle and that glutamate synthesis in these conditions takes place through the coupling of the NADP-linked isocitrate and glutamate dehydrogenases.

Copyright information

© Dr. W. Junk b.v. Publishers 1975

Authors and Affiliations

  • Alberto Machado
    • 1
  • Ignacio Nuñez de Castro
    • 1
  • Federico Mayor
    • 1
  1. 1.Departmento de Bioquímica y Biología MolecularUniversidad AutónomaMadridSpain

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