Chromosoma

, Volume 99, Issue 5, pp 330–335

Meiotic chromosome behaviour reflects levels of sequence divergence inSus scrofa domestica satellite DNA

  • M. Jantsch
  • B. Hamilton
  • B. Mayr
  • D. Schweizer
Article

DOI: 10.1007/BF01731720

Cite this article as:
Jantsch, M., Hamilton, B., Mayr, B. et al. Chromosoma (1990) 99: 330. doi:10.1007/BF01731720

Abstract

We present a general model for the evolution of chromosome-specific satellite DNA subfamilies.Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac “subgenomes”). We show that the centromeric heterochromatin is characterised by two distinct satellite DNA families designed Mc1 and Ac2. Mc1 is a diverse satellite family of the Mc subgenome of which certain members with a 100 bp repeat unit are found to occur at the pericentromeric regions of each Mc autosome, while others are chromosome-specific, e.g. clone Mc pAv1.5, a higher order repeat variant, which hybridises specifically to chromosome 1. Ac2 is a homogeneous satellite occurring at the subterminal pericentromeric regions of all Ac autosomes. DNA sequence analyses showed that all clones investigated are built up from a 14 bp repeat unit which is highly conserved. In situ hybridisation to meiotic pachytene nuclei revealed a distinct spatial arrangement of the Ac2 centromeric satellite.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • M. Jantsch
    • 1
  • B. Hamilton
    • 1
  • B. Mayr
    • 2
  • D. Schweizer
    • 1
  1. 1.Department of Cytology and Genetics, Institute of BotanyUniversity of ViennaViennaAustria
  2. 2.Department of Animal Breeding and GeneticsUniversity of Veterinary MedicineViennaAustria
  3. 3.Department of EmbryologyCarnegie Institution of WashingtonBaltimoreUSA