Chromosoma

, Volume 99, Issue 6, pp 432–435

Determination of sex chromosomal constitution and chromosomal origin of drumsticks, drumstick-like structures, and other nuclear bodies in human blood cells at interphase by fluorescence in situ hybridization

  • Asit B. Mukherjee
  • Nasser Z. Parsa
Article

DOI: 10.1007/BF01726695

Cite this article as:
Mukherjee, A.B. & Parsa, N.Z. Chromosoma (1990) 99: 432. doi:10.1007/BF01726695

Abstract

The sex chromosomal constitution has been determined in various types of human leukocytes at interphase by use of fluorescence in situ hybridization with X- and/or Y-specific DNA probes. It is found that during aging and differentiation of myelocytes into polymorphs there is no significant change in the relative frequency of various types of male and female cells with a specific type of sex chromosomal constitution. Nonrandom variability of the relative proximity between the X chromosomes within the nuclei is also observed in female cells. Moreover, we are the first to determine that sex-specific “drumsticks” and “sessile nodules” in female polymorphs originate from the X chromosomes and that non-sex-specific “drumstick-like” bodies in male polymorphs are of Y chromosomal origin.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Asit B. Mukherjee
    • 1
  • Nasser Z. Parsa
    • 1
  1. 1.Department of Biological SciencesFordham UniversityBronxUSA