European Journal of Epidemiology

, Volume 10, Issue 4, pp 427–434

Is plasmid based differentiation ofCoxiella burnetii in ‘acute’ and ‘chronic’ isolates still valid?

  • D. Thiele
  • H. Willems

DOI: 10.1007/BF01719667

Cite this article as:
Thiele, D. & Willems, H. Eur J Epidemiol (1994) 10: 427. doi:10.1007/BF01719667


Ten humanCoxiella burnetii isolates from french patients with acute hepatitis or chronic endocarditis were characterized according to their polymorphism in DNA restriction patterns and differentiated by plasmid-specific PCR. The aim of this investigation was to clarify if the present classification of so called ‘acute’ and ‘chronic’Coxiella burnetii isolates — based on plasmid profile of a so far limited number of partly ancient isolates — could be confirmed with lately isolated organisms of this agent. The data obtained in this investigation indicate that this classification based onC. burnetii plasmid content is no longer justified.

Key words

Coxiella burnetiiDifferentiationPCRPlasmidPulsed field gel electrophoresisQ fever

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • D. Thiele
    • 1
  • H. Willems
    • 1
  1. 1.Institut für Hygiene und Infektionskrankheiten der TiereJustus-Liebig-Universität GiessenGiessenGermany