, Volume 22, Issue 5, pp 426-433

Inhalation of prostacyclin (PGI2) for 8 hours does not produce signs of acute pulmonary toxicity in healty lambs

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Abstract

Objective

To study the potential side effects and toxicity of inhaling prostacyclin (PGI2) aerosol for 8 h.

Design

In a prospective, randomized study 14 healthy lambs received either PGI2 (n=7) or 0.9% NaCl (n=7) as an aerosol for 8 h.

Setting

Institute for Surgical Research of the Ludwig-Maximilians-University of Munich.

Interventions

All animals were studied under general anesthesia in a prone position. They were first intubated endotracheally and later tracheotomized. PGI2 solution (median dose 28 ng/kg per min) or 0.9% NaCl was administered with a jet nebulizer (delivery rate 4–10 ml/h; mass median diameter of aerosol particles 3.1 μm). Bronchoalveolar lavage was performed before and after the inhalation period to collect epithelial lining fluid of alveoli.

Measurements and results

Hemodynamic and respiratory parameters, systemic resorption (plasma levels of 6-keto-prostaglandin-F1α), in vitro bleeding time, collagen-induced platelet aggregation and global biochemical and cellular composition of the epithelial lining fluid were examined in order to assess the sie effects and signs of acute pulmonary toxicity induced by inhaled PGI2. No statistically significant differences were found between the PGI2 and the control groups for any of the parameters examined.

Conclusion

Inhalation of PGI2 (28 ng/kg per min) over a period of 8 h in healthy lambs does not produce major side effects or acute pulmonary toxicity.

PGI2 (Flolan) was supplied by Wellcome, London, UK
The study was supported, in part, by the Deutsche Forschungsgemeinschaft, grant zw 37/4-1