Intensive Care Medicine

, Volume 22, Issue 11, pp 1169–1175

Serum MIP-1α and IL-8 in septic patients

  • S. Fujishima
  • J. Sasaki
  • Y. Shinozawa
  • K. Takuma
  • H. Kimura
  • M. Suzuki
  • S. Hori
  • N. Aikawa
  • M. Kanazawa
Original

DOI: 10.1007/BF01709331

Cite this article as:
Fujishima, S., Sasaki, J., Shinozawa, Y. et al. Intensive Care Med (1996) 22: 1169. doi:10.1007/BF01709331

Abstract

We studied blood MIP-1α and IL-8 in 38 septic patients and 5 healthy volunteers. Both chemokines were undetectable in the healthy volunteers. In sepsis, serum MIP-1α was detected in 45% of the patients and IL-8 in 84%. The levels of MIP-1α, but not of IL-8, correlated with CRP, IL-6 and TNFα levels. Complication, including various organ failures and mortality, showed no correlation with serum MIP-1α levels. In contrast, we found increased levels of serum IL-8 in septic patients with disseminated intravascular coagulation, central nervous system (CNS) dysfunction or renal failure, and the mortality rate was higher in the IL-8-detectable group than in the IL-8 undetectable group (50% vs 0%,p<0.05). In conclusion, the production of both MIP-1α and IL-8 was increased and initially detectable levels of circulating IL-8 predicted high mortality in sepsis.

Objective

To determine the significance of the C-C chemokine MIP-1α and the C-X-C chemokine IL-8 in sepsis.

Design

Prospective study.

Setting

Clinical investigation, emergency department and general intensive care unit of university hospital.

Patients and participants

38 septic patients and 5 healthy volunteers were studied. Sepsis was diagnosed following the criteria formulated by ACCP/SCCM.

Interventions

10–20 ml of blood was drawn from each patient at the time of initial diagnosis of sepsis.

Measurements and results

MIP-1α and IL-8 were determined by sand-wich ELISA. Both chemokines were undetectable in the healthy volunteers. In sepsis, serum MIP-1α was detected in 45% of the patients and IL-8 was detected in 84%. The levels of MIP-1α, but not of IL-8, correlated with CRP, IL-6 and TNFα levels. Complications, including various organ failures and mortality, showed no correlation with serum MIP-1α levels. In contrast, we found increased levels of serum IL-8 in patients with disseminated intravascular coagulation (DIC) (p<0.05), central nervous system (CNS) dysfunction (p<0.05), renal failure (p<0.01) and the mortality rates were higher in the IL-8 detectable group than in the IL-8 undetectable group (50% vs 0%,p<0.05).

Conclusions

The production of MIP-1α and IL-8 was increased in sepsis. Furthermore, an initially detectable level of circulating IL-8, but not MIP-1α, predicted a high mortality in sepsis diagnosed according to the ACCP/SCCM criteria.

Key words

Macrophage inflammatory protein-1 alpha (MIP-1α) kwInterleukin-8 (IL-8)SepsisDisseminated intravascular Coagulation (DIC)

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • S. Fujishima
    • 1
  • J. Sasaki
    • 1
  • Y. Shinozawa
    • 1
  • K. Takuma
    • 1
  • H. Kimura
    • 1
  • M. Suzuki
    • 1
  • S. Hori
    • 1
  • N. Aikawa
    • 1
  • M. Kanazawa
    • 2
  1. 1.Department of Emergency and Critical Care Medicine, School of MedicineKeio UniversityTokyo 160Japan
  2. 2.Department of Medicine, School of MedicineKeio UniversityTokyo 160Japan