Virus Genes

, Volume 9, Issue 1, pp 53–59

Glycoprotein B of bovine herpesvirus type 4: Its phylogenetic relationship to gB equivalents of the herpesviruses

  • Michael Goltz
  • Hermann Broll
  • Annette Mankertz
  • Wolfgang Weigelt
  • Hanns Ludwig
  • Hans-Jörg Buhk
  • Kerstin Borchers
Article

DOI: 10.1007/BF01703435

Cite this article as:
Goltz, M., Broll, H., Mankertz, A. et al. Virus Genes (1994) 9: 53. doi:10.1007/BF01703435

Abstract

In order to estimate the phylogenetic relationship of BHV-4 among the herpesviruses, we have cloned and sequenced its glycoprotein B (gB). The 2.6 kb open reading frame codes for a 874 amino acid long protein. The comparison of its deduced amino acid sequence with those of its counterparts in 19 distinct herpesviruses groups BHV-4 into the γ-herpesvirinae. The calculation of an evolutionary tree emphasized that BHV-4 is more closely related to herpesvirus saimiri (HVS) than to Epstein-Barr virus (EBV). However, in contrast to EBV and HVS, the gB of BHV-4 contains a putative protease cleavage site and 20 potential N-glycosylation sites. The alignment of the amino acid sequences revealed that 10 cysteine and 7 proline residues, as well as the motifs SPF and GQLG, were completely conserved among the 20 investigated gBs.

Key words

BHV-4 gBγ-herpesvirussequence alignmentconserved domainsevolutionary tree

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Michael Goltz
    • 1
  • Hermann Broll
    • 1
  • Annette Mankertz
    • 1
  • Wolfgang Weigelt
    • 1
  • Hanns Ludwig
    • 2
  • Hans-Jörg Buhk
    • 1
  • Kerstin Borchers
    • 2
  1. 1.Robert Koch-InstitutBerlinGermany
  2. 2.Institut für Virologie der Freien Universität BerlinBerlinGermany