Virus Genes

, Volume 9, Issue 3, pp 231–245

Two types of deletions in orthopoxvirus genomes

  • Sergei Nikolaevich Shchelkunov
  • Alexei Vladimirovich Totmenin
Article

DOI: 10.1007/BF01702879

Cite this article as:
Shchelkunov, S.N. & Totmenin, A.V. Virus Genes (1995) 9: 231. doi:10.1007/BF01702879

Abstract

The genome nucleotide sequences of two strains of variola major virus and one strain of vaccinia virus were compared. One hundred and sixty-eight short (less than 100 bp in length) and eight long (more than 900 bp in length) deletions, four deletion/insertion regions, and four regions of multiple mutational differences between variola and vaccinia virus DNAs were revealed. Short deletions generally occur at directly repeated sequences of 3–21 bp. Long deletions showed no evidence of repeated sequences at their points of junction. We suggest the presence of a consensus sequence characteristic of these junctions and propose that there is a virus-encoded enzyme that produces this nonhomologous recombination/deletion in the cytoplasm of the infected cell.

Key words

variola virus vaccinia virus genome analysis repeated sequences deletions 

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Sergei Nikolaevich Shchelkunov
    • 1
  • Alexei Vladimirovich Totmenin
    • 1
  1. 1.Institute of Molecular BiologyRussian State Research Center NPO “Vector”Koltsovo, Novosibirsk regionRussia