Annals of Hematology

, Volume 69, Issue 6, pp 297–302

Improvement of hematologic recovery after high-dose intensification using peripheral blood progenitor cells (PBPC) mobilized by chemotherapy and GM-CSF

Authors

  • T. Lamy
    • Service d'hématologie cliniqueHopital Pontchaillou
  • B. Drenou
    • Laboratoire d'immuno-hématologieHopital de Rennes
  • I. Grulois
    • Service d'hématologie cliniqueHopital Pontchaillou
  • C. Leberre
    • Centre régional de transfusion sanguineHopital de Rennes
  • C. Dauriac
    • Service d'hématologie cliniqueHopital Pontchaillou
  • L. Amiot
    • Laboratoire d'immuno-hématologieHopital de Rennes
  • M. Godard
    • Laboratoire d'immuno-hématologieHopital de Rennes
  • R. Fauchet
    • Laboratoire d'immuno-hématologieHopital de Rennes
  • P. Y. LePrise
    • Service d'hématologie cliniqueHopital Pontchaillou
Original Article

DOI: 10.1007/BF01696558

Cite this article as:
Lamy, T., Drenou, B., Grulois, I. et al. Ann Hematol (1994) 69: 297. doi:10.1007/BF01696558

Summary

We have tested the efficiency of GM-CSF to mobilize peripheral blood progenitor cells (PBPC) and evaluated the hematological reconstitution after GM-CSF primed-PBPC infusion following myeloablative therapy. Twenty three patients suffering from hematological malignancies were included in this study. Starting 24 hours after completion of a standard dose chemotherapy including vindesine, cyclophosphamide, adriblastine, prednisone, (VCAP), 5 (μg/kg sub-cutaneous daily dose GM-CSF was given for a median time of 14 days followed by three consecutives cycles of leukapheresis. Fifteen of these 23 patients underwent GM-CSF primed-PBPC autotransplantation following high dosed intensification regimen. PBPC collection and hematopoietic recovery were compared with a 15 patients control group who did not receive GM-CSF. No marrow or growth factors were administred after PBPC reinfusion in the two groups. VCAP/GM-CSF mobilization induced significantly higher yields of CFU-GM (3.8 fold) than did VCAP mobilization alone, 19×104/kg (2–73) vs 5×104/kg (2–27), (p<0.005). The median number of days to achieve 1.109/l neutrophils, platelet count >20.109/l and >50.109/l was significantly lower in the GM-CSF group than in the control group, respectively 13 vs 19 days (p=0.04), 15.5 vs 27 days (p<0.02), 19 vs 51 days (p<0.01). When compared with the control group, transfusion requirements and median of hospital stay were both significantly decreased for the patients receiving GM-CSF primed-PBPC. Our study confirms that infusion of GM-CSF primed-PBPC as a sole source of hematopoietic support improves hematopoietic reconstitution following myeloablative therapy.

Key words

GM-CSFAutologous blood stem cells Transplantation

Copyright information

© Springer-Verlag 1994