Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever
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- D'Harcourt, S.C., Soto, A.B., Burgos, V.C. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1996) 15: 749. doi:10.1007/BF01691965
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To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.