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European Journal of Clinical Microbiology and Infectious Diseases

, Volume 15, Issue 9, pp 749-752

First online:

Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

  • S. Cruchaga D'HarcourtAffiliated withMicrobiology Department, La Paz Universitary Hospital
  • , A. Buño SotoAffiliated withMicrobiology Department, La Paz Universitary Hospital
  • , V. Cascante BurgosAffiliated withMicrobiology Department, La Paz Universitary Hospital
  • , D. Lozano CaleroAffiliated withMicrobiology Department, La Paz Universitary Hospital
  • , R. Martínez-ZapicoAffiliated withMicrobiology Department, La Paz Universitary Hospital

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Abstract

To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.