European Journal of Clinical Microbiology and Infectious Diseases

, Volume 15, Issue 9, pp 749–752

Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

  • S. Cruchaga D'Harcourt
  • A. Buño Soto
  • V. Cascante Burgos
  • D. Lozano Calero
  • R. Martínez-Zapico
Notes

DOI: 10.1007/BF01691965

Cite this article as:
D'Harcourt, S.C., Soto, A.B., Burgos, V.C. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1996) 15: 749. doi:10.1007/BF01691965
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Abstract

To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.

Copyright information

© MMV Medizin Verlag GmbH 1996

Authors and Affiliations

  • S. Cruchaga D'Harcourt
    • 1
  • A. Buño Soto
    • 1
  • V. Cascante Burgos
    • 1
  • D. Lozano Calero
    • 1
  • R. Martínez-Zapico
    • 1
  1. 1.Microbiology DepartmentLa Paz Universitary HospitalMadridSpain