Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test
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- Nüesch-Inderbinen, M.T., Hächler, H. & Kayser, F.H. Eur. J. Clin. Microbiol. Infect. Dis. (1996) 15: 398. doi:10.1007/BF01690097
A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum β-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of theblaSHV genes, followed by restriction withNheI. Due to the glycine (position 238) (SHV-non-ESBL) → serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carryingblaSHV-ESBL genes as ESBL producers.