European Journal of Clinical Microbiology and Infectious Diseases

, Volume 15, Issue 5, pp 398–402

Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test

  • M. T. Nüesch-Inderbinen
  • H. Hächler
  • F. H. Kayser
Notes

DOI: 10.1007/BF01690097

Cite this article as:
Nüesch-Inderbinen, M.T., Hächler, H. & Kayser, F.H. Eur. J. Clin. Microbiol. Infect. Dis. (1996) 15: 398. doi:10.1007/BF01690097

Abstract

A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum β-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of theblaSHV genes, followed by restriction withNheI. Due to the glycine (position 238) (SHV-non-ESBL) → serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carryingblaSHV-ESBL genes as ESBL producers.

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. T. Nüesch-Inderbinen
    • 1
  • H. Hächler
    • 1
  • F. H. Kayser
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of ZürichZürichSwitzerland