European Journal of Clinical Microbiology and Infectious Diseases

, Volume 17, Issue 2, pp 85–89

Increasing incidence of meningococcal disease in Spain associated with a new variant of serogroup C

  • S. Berrón
  • L. De La Fuente
  • E. Martín
  • J. A. Vázquez
Article

DOI: 10.1007/BF01682161

Cite this article as:
Berrón, S., De La Fuente, L., Martín, E. et al. Eur. J. Clin. Microbiol. Infect. Dis. (1998) 17: 85. doi:10.1007/BF01682161

Abstract

Serogroup B has been the main cause of meningococcal disease in Spain since at least 1979, but in recent years an increase in the prevalence of infection due to serogroup C meningococci has been detected. In 1996, for the first time, most cases of meningococcal disease were caused by serogroup C strains. The sero/subtype of all serogroup C meningococci received from 1993 to June 1996 was determined, and the results showed that C∶2b∶P1.2,5, the most common phenotype in 1995 and 1996 (63% and 65%, respectively), represented only 4.8% of strains in 1993. The C∶2b∶P1.2,5 epidemic strains appear to be responsible for the high prevalence of serogroup C in Spain. One hundred fifty-one randomly selected serogroup C strains were analyzed by multilocus enzyme electrophoresis, ribotyping, and pulsedfield gel electrophoresis. Pulsed-field gel electrophoresis provided the most accurate information: more than 80% of the C∶2b∶P1.2,5 and C∶2b∶P1.2 isolates exhibited one of two very closely related profiles, while most of the C:2b:NST and C∶2b∶P1.5 strains had a pattern located at a genetic distance of 0.24 from those two profiles. The results show that C∶2b∶P1.2,5 strains represent a subclone or a genetic variant of the previously identified Spanish epidemic clone C∶2b∶non-subtypable strains.

Key words

MeningococciGenotypingClonality

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • S. Berrón
    • 1
  • L. De La Fuente
    • 1
  • E. Martín
    • 1
  • J. A. Vázquez
    • 1
  1. 1.Laboratorio de Referenda de Meningococos - Servicio de BacteriologíaCentro Nacional de Microbiología, Instituto de Salud Carlos IIIMajadahonda, MadridSpain