Molecular Breeding

, Volume 1, Issue 1, pp 51–63

Mitochondrial DNA polymorphism and phylogenetic relationships inHevea brasiliensis

  • Hong Luo
  • Benoît Van Coppenolle
  • Marc Seguin
  • Marc Boutry
Article

DOI: 10.1007/BF01682089

Cite this article as:
Luo, H., Van Coppenolle, B., Seguin, M. et al. Mol Breeding (1995) 1: 51. doi:10.1007/BF01682089

Abstract

Using fourteen random mitochondrial DNA probes, we have examined restriction fragment length polymorphism (RFLP) in wild and cultivatedHevea brasiliensis. A total of 395 accessions, including 345 from various prospectings collected in Brazil, Colombia and Peru and 50 cultivated clones, were analyzed. Two other species (H. benthamiana andH. pauciflora) were also included in the study for comparison. The high level of mitochondrial polymorphism allowed us to divide all the accessions analyzed into 212 distinct genotypes. The genetic variability of cultivated clones was limited to four genotypes forming two clusters. In contrast, considerable genetic variation was found in the wild collections. In almost all cases, accessions displaying the same RFLP profile were restricted to the same geographical area (same or neighbor administrative districts). In addition, accessions whose genetic closeness was predicted by RFLP profiles were also clustered according to geographical origin. In a few cases, however, similar RFLP profiles were found for accessions originating from geographically distant districts. This discrepancy can be explained either by seed dispersion (by river) or possibly by similar genetic events occurring independently in different geographical locations. Chloroplast DNA RFLP was also analyzed in 217 accessions, representative of 126 distinct mitochondrial genotypes. Very few differences were found, indicating that the chloroplast genome is more highly conserved than the mitochondrial genome.

Key words

Hevea brasiliensis rubber tree mitochondrial DNA chloroplast DNA restriction fragment length polymorphism phylogeny 

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Hong Luo
    • 1
  • Benoît Van Coppenolle
    • 1
  • Marc Seguin
    • 2
  • Marc Boutry
    • 1
  1. 1.Unité de Biochimie PhysiologiqueUniversité Catholique de LouvainLouvain-la-NeuveBelgium
  2. 2.CIRAD (Perennial Crops Department)BIOTROPMontpellierFrance

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