Virchows Archiv A

, Volume 421, Issue 4, pp 315–322

An immunohistochemical study of the breast using antibodies to basal and luminal keratins, alpha-smooth muscle actin, vimentin, collagen IV and laminin

Part I: normal breast and benign proliferative lesions
  • Werner Böcker
  • Bert Bier
  • Götz Freytag
  • Bettina Brömmelkamp
  • Ernst-Dieter Jarasch
  • Georg Edel
  • Barbara Dockhorn-Dworniczak
  • Kurt W. Schmid
Original Articles

DOI: 10.1007/BF01660978

Cite this article as:
Böcker, W., Bier, B., Freytag, G. et al. Vichows Archiv A Pathol Anat (1992) 421: 315. doi:10.1007/BF01660978

Summary

The distribution of simple epithelial (K8/18/ 19) and basal (myoepithelial) (K5/14) keratins, α-smooth-muscle actin, vimentin, collagen IV and laminin in normal mammary glands and in benign proliferative lesions was studied using monoclonal antibodies (mAbs). These antibodies (Abs) identified myoepithelial cells and luminal cells specifically. In lesions with adenosis and papillomas, the two-layered formation resembled that of normal glands with a purely myoepithelial-epithelial differentiation. In scleradenotic lesions, the main cell was of myoepithelial immunophenotype with intermixed trabecular-tubular proliferations of simple-type epithelium. The sclerosis seems to be the result of an irregular basal lamina synthesis by the myoepithelial cells. In contrast to these lesions, epitheliosis represents a purely intraluminal cell proliferation of clearly simple epithelial immunophenotype and of cells with a basal keratin phenotype, lacking myoepithelial differentiation antigen actin. The basal keratin type epithelium may represent post-stem or intermediate cells developing into luminal epithelium. Epitheliosis appears to be a purely epithelial hyperplasia with striking similarity to the regeneration of normal breast epithelium. The different proliferative patterns may give an explanation for differences in potential cancer risks of patients with these lesions.

Key words

Hyperplastic breast lesions Anti-keratin antibody Anti-smooth muscle actin antibody Anti-vimentin antibody Anti-collagen IV antibody Immunohistology 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Werner Böcker
    • 1
  • Bert Bier
    • 1
  • Götz Freytag
    • 2
  • Bettina Brömmelkamp
    • 1
  • Ernst-Dieter Jarasch
    • 2
  • Georg Edel
    • 1
  • Barbara Dockhorn-Dworniczak
    • 1
  • Kurt W. Schmid
    • 1
  1. 1.Gerhard Domagk Institute of PathologyUniversity of MünsterMünsterFederal Republic of Germany
  2. 2.Institute of Cell and Tumour Biology, German Cancer Research CentreUniversity of HeidelbergHeidelbergFederal Republic of Germany