Journal of Molecular Evolution

, Volume 1, Issue 3, pp 230–240

Characterization of the primary structural homology between the 16 S ribosomal RNAs ofEscherichia coli andBacillus megaterium by oligomer cataloging

  • Kenneth J. Pechman
  • Carl R. Woese
Article

DOI: 10.1007/BF01660242

Cite this article as:
Pechman, K.J. & Woese, C.R. J Mol Evol (1972) 1: 230. doi:10.1007/BF01660242

Summary

The 16 S ribosomal RNAs of twoProcaryotes, Escherichia coli andBacillus megaterium were characterized by oligomer cataloging (oligomers produced by T1 nuclease digestion), in an attempt to detect their primary structural homology and as an initial step in characterizing this homology. Oligomer sequence coincidence between the two catalogs far in excess of the random expected levels was observed. Statistically significant coincidence was most pronounced for the hexamers and pentamers, suggesting that the overall structure of 16 S ribosomal RNA may be such that conservation of large stretches of its primary structure (e.g. over eight nucleotides in length) is not in general essential.

Key words

Escherichia coliBacillus megateriumOligomer CatalogMolecular Fossil RecordTwo Dimensional ElectrophoretogramSequence HomologyPhylogeny

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Kenneth J. Pechman
    • 1
  • Carl R. Woese
    • 1
  1. 1.Department of MicrobiologyUniversity of llinoisUrbanaUSA