Journal of Neurocytology

, Volume 16, Issue 5, pp 701–711

Expression of growth factor receptors in injured nervous tissue. II. Induction of specific platelet-derived growth factor binding in the injured PNS is associated with a breakdown in the blood-nerve barrier and endoneurial interstitial oedema

  • Gennadij Raivich
  • Georg W. Kreutzberg

DOI: 10.1007/BF01637661

Cite this article as:
Raivich, G. & Kreutzberg, G.W. J Neurocytol (1987) 16: 701. doi:10.1007/BF01637661


We have studied the expression of the platelet-derived growth factor (PDGF) receptors in the injured chick PNS using [125I]-iodinated PDGF as a radioactive probe to map autoradiographically thein situ distribution of specific [125I]PDGF binding.

Crush or transection of the sciatic nerve led to a rapid and massive induction of specific [125I]PDGF binding on fibroblast-like cells of the injured endoneurium, already observed 2 h postoperatively. It is initially characterized by a symmetrical appearance both below and above the site of injury, spreading throughout the distal part of the lesioned nerve 1 to 2 days postoperatively. Comparison with distribution of specific [125I]β-nerve growth factor (β-NGF) binding (see preceding paper) revealed a number of important differences: unlike the specific [125I]β-NGF binding, which rapidly disappears after reinnervation of the distal nerve, this was not observed in the case of [125I]PDGF binding. [125I]PDGF binding also correlated poorly with the extent of axonal injury. The segmental removal of the perineurium, resulting in heavy interstitial oedema without widespread axonal injury, led to a strong, local induction of [125I]PDGF binding, while causing moderate β-NGF binding to only the few degenerating nerve fibre tubes. These results suggest the existence of different pathophysiological mechanisms that regulate the expression of PDGF and β-NGF receptors in the lesioned and regenerating PNS.

Copyright information

© Chapman and Hall Ltd. 1987

Authors and Affiliations

  • Gennadij Raivich
    • 1
  • Georg W. Kreutzberg
    • 1
  1. 1.Department of NeuromorphologyMax-Planck Institute for PsychiatryMartinsriedGermany