Virchows Archiv A

, Volume 422, Issue 4, pp 277–283

Fibre type specific expression of Leu19-antigen and N-CAM in skeletal muscle in various stages after experimental denervation

  • Wolfgang Müller-Felber
  • Klaus Küllmer
  • Petra Fischer
  • Carl-Detlev Reimers
  • Stefan Wagner
  • Ullrich Harland
  • Martin Schmidt-Achert
  • Dieter Pongratz
Original Articles

DOI: 10.1007/BF01608336

Cite this article as:
Müller-Felber, W., Küllmer, K., Fischer, P. et al. Vichows Archiv A Pathol Anat (1993) 422: 277. doi:10.1007/BF01608336

Abstract

Leu-19 antigen, which seems to be identical with neural cell adhesion molecule (N-CAM), plays a major role in the innervation of muscle cells, and in adult muscle appears after denervation and during regeneration of muscle fibres, where it acts as part of a signalling system increasing the probability of re-innervation. This combined enzyme-histochemical and immunohistochemical study examined whether this signalling process was regulated in a uniform or differential pattern for type 1 and type 2 muscle fibres. The subscapular nerve of 18 rabbits was transsected with subsequent complete denervation of the supraspinatus muscle. Leu19 and N-CAM immunohistochemistry was performed 2 to 64 days after surgery. Whereas in normal muscle there are virtually no Leu-19/N-CAM positive muscle fibres; from day 2 after denervation an increasing proportion of fibres expressed Leu-19/N-CAM, prior to any neurogenic atrophy. In the early stage of denervation Leul9/N-CAM expression was confined to type 1 fibres. After 11 days nearly all fibres were Leul9/N-CAM positive irrespective of their fibre type. Sixty-four days after denervation type 1 fibres became Leu19/N-CAM negative, while atrophic type 2 fibres showed intensive staining. Thus, expression of Leu-19 antigenity is differently regulated in both fibre types.

Key words

Neural cell adhesion moleculeLeu19DenervationRabbit

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Wolfgang Müller-Felber
    • 1
  • Klaus Küllmer
    • 2
  • Petra Fischer
    • 1
  • Carl-Detlev Reimers
    • 1
  • Stefan Wagner
    • 1
  • Ullrich Harland
    • 2
  • Martin Schmidt-Achert
    • 1
  • Dieter Pongratz
    • 1
  1. 1.Friedrich Baur Institut bei der Medizinischen Klinik und bei der Neurologischen Klinik, Klinikum InnenstadtLudwig Maximilians UniversitätMunichGermany
  2. 2.Orthopädische Universitätsklinik, Universität GiessenGiessenGermany
  3. 3.Friedrich-Baur-Institut, Klinikum InnenstadtUniversity of MunichMünchen 2Germany