Current Microbiology

, Volume 16, Issue 2, pp 61–67

Inhibitory factors involved in acetone-butanol fermentation byClostridium saccharoperbutylacetonicum

Authors

  • Bhupinder K. Soni
    • Biochemical Engineering Research CenterIndian Institute of Technology
  • Kumudeswar Das
    • Biochemical Engineering Research CenterIndian Institute of Technology
  • Tarun K. Ghose
    • Biochemical Engineering Research CenterIndian Institute of Technology
Article

DOI: 10.1007/BF01588173

Cite this article as:
Soni, B.K., Das, K. & Ghose, T.K. Current Microbiology (1987) 16: 61. doi:10.1007/BF01588173

Abstract

Studies carried out onClostridium saccharoperbutylacetonicum (ATCC 27022) reveal that intracellular and extracellular inhibitors, including metabolic end-products, caused the inhibition of cell growth and solvent production. Butanol at the level of 13.0 g/liter was completely inhibitory to the growth of cells, whereas butyric acid totally inhibited the cell growth at a concentration of 8.7 g/liter. Investigations carried out on the effect of addition of culture filtrate concentrate and cell-free extract concentrate indicate that nonvolatile inhibitors produced by cells were also inhibitory for bioconversion. The butanol production was found to be reduced by 15%–20% on addition of cell-free extract concentrate. Inhibition by concentrates was enhanced in the presence of butanol. Addition of heat-sterilized concentrates resulted in a reduction of inhibition.

Copyright information

© Springer-Verlag New York Inc 1987