Fermentation and toxin studies of the molluscicidal strains ofBacillus brevis
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Strain SS86-4 was one of 40Bacillus brevis strains shown to be molluscicidal to the schistosomiasis snail vectorBiomphalaria glabrata. When grown in mB4 medium in 2-L fermentors, SS86-4 was molluscicidal only if fructose or phenylalanine was present in the medium. This is reminiscent of secondary fermentation factor effects, in this case an antioxidant effect. In vivo proteases also were capable of reducing molluscicidal activity. The molluscicidal toxin has an LC50 of 1 μg toxin protein ml−1 (approx. 1 p.p.m.) and may be described as a small proteinaceous, heat-stable, oxygen-sensitive entity associated with the particulate portion of the cell wall fraction ofB. brevis that is formed prior to sporulation. Initial information indicates that its HPLC signature shows major peaks at 148.37 and 163.96 s and consists of two bands of approximately 5.3 kDa and 8.7 kDa on PAGE gel.
- Agathos, S.N. and A.L. Demain. 1983. Optimization of fermentation processes through the control of in vivo inactivation of microbial biosynthetic enzymes. In: Foundations of Biochemical Engineering (Blanch, H.W., E.T. Papoutsakis and G. Stephanopoulos, eds), pp. 53–67, American Chemical Society, Washington, DC.
- Agathos, S.N. and A.L. Demain. 1986. Substrate amino acid-mediated stabilization of gramicidin S synthetase activity against inactivation in vivo. Enzyme Microb. Technol. 8: 465–468.
- Baumann, P., M.A. Clark, L. Baumann and A.H. Broadwell. 1991.Bacillus sphaericus as a mosquito pathogen: properties of the organism and its tovins. Bacteiol. Rev. 55: 425–436.
- Bone, L.W., K.P. Bottjer and S.S. Gill. 1986.Trichostrongylus colubriformis: isolation and characterization of ovicidal activity fromBacillus thuringiensis isrealensis. Exp. Parasitology 62: 247–253.
- Bradford, M.M. 1976. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
- Cheng, T.C. 1974. Molluscicides in Schistosomiasis Control. Academic Press, New York.
- Davidson, E.W. 1983. Alkaline extraction of toxin from spores of the mosquito pathogen,Bacillus sphaericus strain 1593. Can. J. Microbiol. 29: 271–275.
- Kreig, A. and H.G. Miltenburger. 1984. Bioinsecticides: 1.Bacillus thuringiensis. Adv. Biotechnol. Proc. 3: 273–290.
- Luthy, P., H.R. Ebersold, J.L. Cordier and H.M. Fischer. 1985. Insecticidal metabolites of spore-forming bacilli. In: Fundamental and Applied Aspects of Bacterial Spores (Dring, G.J., D.J. Ellar and G.W. Gould, eds), pp. 475–484, Academic Press, London.
- Margalith, P. 1964. Secondary factors in fermentation processes. Adv. Appl. Microbiol. 6: 69–90.
- McCullogh, F.S. and K.E. Mott. 1983. The role of molluscicides in schistosomiasis control. Document WHO/SCHISTO/83.72.
- Myers, P.S. and A.A. Youston. 1980. Localization of a mosquito-larval toxin ofBacillus sphaericus 1593. Appl. Environ. Microbiol. 39: 1205–1211.
- Schagger, H. and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368–379.
- Singer, S. 1979. Use of entomogenous bacteria against insects of public health importance. Devel. Indust. Microbiol. 20: 117–122. Society for Industrial Microbiology, Arlington, Virginia.
- Singer, S. 1987. Current status of the microbial larvicideBacillus sphaericus. In: Biotechnology Advances in Insect Pathology and Cell Culture (Maramorosch, K., ed.), pp. 133–163, Academic Press, New York.
- Singer, S., K.A. Doherty and A.D. Stambaugh. 1988. Preliminary examination of molluscicidal strains of the genus Bacillus. 88th Annual Meeting American Society for Microbiology, Miami, Florida (Abstr., p. 76).
- Stecher, P.G., M.J. Finkel, O.H. Siegmund and B.M. Zafranski. 1960. The Merck Index of Chemicals and Drugs, p. 497, Merck and Co. Inc., Rahway.
- Williston, B.K. and S. Singer. 1987. Initial studies of the use of aminopeptidases for the differentiation ofBacillus sphaericus strains. J. Ind. Microbiol. 2: 285–292.
- World Health Organization. 1984. Report of an informal consultation on research on the biological control of snail intermediate hosts. Document TDR/BCV-SCH/SIH/84.3, Geneva.
- Fermentation and toxin studies of the molluscicidal strains ofBacillus brevis
Journal of Industrial Microbiology
Volume 13, Issue 2 , pp 112-119
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- MolluscicidalBacillus toxin
- Bacillus brevis
- Biomphalaria glabrata
- Biocontrol of snails
- Antioxidant preservation of toxin
- Secondary fermentation factor
- Industry Sectors