Journal of Industrial Microbiology

, Volume 11, Issue 2, pp 73–81

Screening of microbial esterases for asymmetric hydrolysis of 2-ethylhexyl butyrate

  • Ching T. Hou

DOI: 10.1007/BF01583678

Cite this article as:
Hou, C.T. Journal of Industrial Microbiology (1993) 11: 73. doi:10.1007/BF01583678


A pH indicator agar plate method was used to screen for esterase activities for hydrolysis of 2-ethylhexyl butyrate. Seven hundred and fifty-seven selected microbial cultures, including 325 bacteria, and 432 yeasts and actinomycetes from the ARS Culture, Collection, were screened. Among them, 62 cultures hydrolyzed 2-ethylhexyl butyrate. Of these strains only 17 showed lipase activity on a rhodamine B lipase screen. The reaction products, 2-ethyl-1-hexanol andn-butyric acid were confirmed by gas-liquid chromatography (GC) and GC/MS analyses. The yield of 2-ethyl-1-hexanol varied depending on the strains of the microorganisms, with the highest yield at 79.1% by a strain ofPseudomonas myxogenes Product analyses with a cyclodextrin GC chiral column showed that two strains ofPseudomonas produced, greater than 80% enantiomeric excess of S(+)-2-ethyl-1-hexanol.

Key words

EsteraseEthylhexyl butyrateHydrolytic stereospecificity

Copyright information

© Society for Industrial Microbiology 1993

Authors and Affiliations

  • Ching T. Hou
    • 1
  1. 1.Oil Chemical Research, National Center for Agricultural Utilization Research, Agricultural Research ServiceUS Department of AgriculturePeoriaUSA