Suspension culture ofAedes albopictus cells for flavivirus mass production
- Cite this article as:
- Morita, K. & Igarashi, A. Journal of Tissue Culture Methods (1989) 12: 35. doi:10.1007/BF01578006
Aedes albopictus clone C6/36 cells were adapted to suspension culture in spinner flasks, and largescale cell cultures were achieved. Suspension cultured cells, attached on microcarriers, were inoculated with Japanese encephalitis virus. Virus-infected fluid with 107 to 108 plaque-forming units/ml was harvested daily from Days 2 to 8 postinfection. This method makes it possible to prepare large amounts of purified virions with less culture space and labor.