, Volume 12, Issue 1, pp 35-37

Suspension culture ofAedes albopictus cells for flavivirus mass production

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Summary

Aedes albopictus clone C6/36 cells were adapted to suspension culture in spinner flasks, and largescale cell cultures were achieved. Suspension cultured cells, attached on microcarriers, were inoculated with Japanese encephalitis virus. Virus-infected fluid with 107 to 108 plaque-forming units/ml was harvested daily from Days 2 to 8 postinfection. This method makes it possible to prepare large amounts of purified virions with less culture space and labor.