Journal of tissue culture methods

, Volume 12, Issue 1, pp 35–37

Suspension culture ofAedes albopictus cells for flavivirus mass production

  • Kouichi Morita
  • Akira Igarashi
Article

DOI: 10.1007/BF01578006

Cite this article as:
Morita, K. & Igarashi, A. Journal of Tissue Culture Methods (1989) 12: 35. doi:10.1007/BF01578006

Summary

Aedes albopictus clone C6/36 cells were adapted to suspension culture in spinner flasks, and largescale cell cultures were achieved. Suspension cultured cells, attached on microcarriers, were inoculated with Japanese encephalitis virus. Virus-infected fluid with 107 to 108 plaque-forming units/ml was harvested daily from Days 2 to 8 postinfection. This method makes it possible to prepare large amounts of purified virions with less culture space and labor.

Key words

suspension culture Aedes albopictus cells flaviviruses 

Copyright information

© Tissue Culture Association, Inc 1989

Authors and Affiliations

  • Kouichi Morita
    • 1
  • Akira Igarashi
    • 1
  1. 1.Department of Virology, Institute of Tropical MedicineNagasaki UniversityNagasakiJapan

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