Current Microbiology

, Volume 26, Issue 2, pp 113–116

A membrane technique for producing protoplasts ofCryphonectria parasitica


  • Jyan-Chyun Jang
    • Department of Forest ResourcesClemson University
  • Susan D. McElreath
    • Department of Forest ResourcesClemson University
  • Frank H. Tainter
    • Department of Forest ResourcesClemson University

DOI: 10.1007/BF01577346

Cite this article as:
Jang, J., McElreath, S.D. & Tainter, F.H. Current Microbiology (1993) 26: 113. doi:10.1007/BF01577346


A method for the formation and regeneration of protoplasts of several strains of the chestnut blight fungus,Cryphonectria parasitica, is presented. The procedure utillizes cellophane membranes for growth and employs centrifugation for separation of protoplasts from hyphal fragments. Yields averaged 8.04×106 protoplasts per membrane. Regeneration frequencies were 40–50% with a soft-agar overlay. These protoplasts are suitable for use in experiments designed to determine the role of dsRNA in hypovirulence ofC. parasitica.

Copyright information

© Springer-Verlag New York Inc. 1993