Current Microbiology

, Volume 24, Issue 5, pp 275–280

Inorganic phosphate transport inAcinetobacter lwoffi

  • Jacob Yashphe
  • Hemant Chikarmane
  • Maria Iranzo
  • Harlyn O. Halvorson
Article

DOI: 10.1007/BF01577332

Cite this article as:
Yashphe, J., Chikarmane, H., Iranzo, M. et al. Current Microbiology (1992) 24: 275. doi:10.1007/BF01577332

Abstract

The transport of inorganic phosphate has been studied inAcinetobacter lwoffi JW11. During growth on excess phosphate, only one transport system was present, with an apparent Km of 1.4 μM. When cells were starved for phosphate, a second uptake system with an apparent Km of 110 nM was also synthesized. The two transport systems could be distinguished by differing sensitivities to the phosphate analogs arsenate and 2-aminoethylphosphonate. Both systems were inhibited by carbonylcyanidem-chlorophenylhydrazone, and to a lesser extent by Na azide. The high-affinity transport system was inactivated by osmotic shock treatment and by spheroplast formation. Preliminary evidence for a phosphate-binding protein in the osmotic shock fluid is presented. The isolation of a mutant constitutive for the high-affinity transport system is described.

Copyright information

© Springer-Verlag New York Inc. 1992

Authors and Affiliations

  • Jacob Yashphe
    • 1
  • Hemant Chikarmane
    • 1
  • Maria Iranzo
    • 1
  • Harlyn O. Halvorson
    • 1
  1. 1.Marine Biological LaboratoryWoods HoleUSA
  2. 2.Department of BacteriologyThe Hebrew University, Hadassah Medical SchoolJerusalemIsrael

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