Journal of Industrial Microbiology

, Volume 3, Issue 3, pp 139–146

Filamentous growth ofPseudomonas aeruginosa

  • J. Barry Wright
  • J. W. Costerton
  • W. F. McCoy
Original Papers

DOI: 10.1007/BF01569520

Cite this article as:
Wright, J.B., Costerton, J.W. & McCoy, W.F. Journal of Industrial Microbiology (1988) 3: 139. doi:10.1007/BF01569520

Summary

The growth of two strains ofPseudomonas aeruginosa in stirred batch cultures was monitored by optical density, DNA concentration, and acridine orange direct cell count measurements. Growth of adherent bacteria in pure culture was also observed on suspended glass discs by light and scanning electron microscopy. Strain MUCOID produced significant numbers of filamentous cells in broth culture and in the adherent population, while strain PAO 381 did not produce elongated cells. Filamentous growth of MUCOID could be prevented by the addition of 5 × 10−2 M Mg2+. However, the addition of 0.66 mM EDTA caused an increased proportion of the population (>50%) of MUCOID cells to become filamentous in broth culture. The results are discussed and related to theories regarding bacterial plasticity, and filamentation of normally bacillary cells.

Key words

BiofilmPlasticityAdherent bacteriaElongation

Copyright information

© Society for Industrial Microbiology 1988

Authors and Affiliations

  • J. Barry Wright
    • 1
  • J. W. Costerton
    • 1
  • W. F. McCoy
    • 1
  1. 1.Department of Biological SciencesUniversity of CalgaryCalgaryCanada
  2. 2.Great Lakes Chemical CorporationWest LafayetteUSA