Journal of Industrial Microbiology

, Volume 2, Issue 2, pp 87–95

Effects of fermentation feeding strategies prior to induction of expression of a recombinant malaria antigen inEscherichia coli

Authors

  • Dane W. Zabriskie
    • Biological Process SciencesSmith Kline & French Laboratories
  • Dave A. Wareheim
    • Biological Process SciencesSmith Kline & French Laboratories
  • Michael J. Polansky
    • Biological Process SciencesSmith Kline & French Laboratories
Original Papers

DOI: 10.1007/BF01569507

Cite this article as:
Zabriskie, D.W., Wareheim, D.A. & Polansky, M.J. Journal of Industrial Microbiology (1987) 2: 87. doi:10.1007/BF01569507

Summary

A variety of feeding strategies have been described for attaining high cell densities in fed-batch fermentors. Although cell density is an important component in the produtivity of recombinant fermentations, it must be achievable with high product expression levels. Experiments were conducted to study the influence of fermentation feeding strategies on the production of a recombinant malaria antigen inEscherichia coli. C-source feeding profiles were calculated to maintain specific growth rates at 0.1, 0.2, 0.35, and 0.5 l/h prior to induction in defined and complex media using an exponential growth model. Fed-batch fermentations employing these feeding profiles effectively controlled the specific growth rates prior to induction. Antigen yields per dry cell weight did not vary with specific growth rate. Antigen yields from fed-batch fermentations achieving high cell densities were similar to batch fermentations achieving low cell densities. These results show that C-feeding policies can limit growth without reducing expression levels in some systems, and suggest applications in managing oxygen demand and catabolic by-product formation during process scale-up.

Key words

FermentationProcess controlExpression of recombinant proteinsEscherichia coliMalaria vaccine

Copyright information

© Society for Industrial Microbiology 1987