The relationship between hydrogen metabolism, sulfate reduction and nitrogen fixation in sulfate reducers
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Hydrogenase and nitrogenase activities of sulfate-reducing bacteria allow their adaptation to different nutritional habits even under adverse conditions. These exceptional capabilities of adaptation are important factors in the understanding of their predominant role in problems related to anaerobic metal corrosion. Although the D2−H+ exchange reaction indicated thatDesulfovibrio desulfuricans strain Berre-Sol andDesulfovibrio gigas hydrogenases were reversible, the predominant activity in vivo was hydrogen uptake. Hydrogen production was restricted to some particular conditions such as sulfate or nitrogen starvation. Under diazotrophic conditions, a transient hydrogen evolution was followed by uptake when dinitrogen was effectively fixed. In contrast, hydrogen evolution proceeded when acetylene was substituted as the nitrogenase substrate. Hydrogen can thus serve as an electron donor in sulfate reduction and nitrogen metabolism.
- Badziong, W., R.K. Thauer and J.G. Zeikus. 1978. Isolation and characterization ofDesulfovibrio growing on hydrogen plus sulfate as the sole energy source. Arch. Microbiol. 116: 41–49.
- Berlier, Y.M., B. Dimon, G. Fauque and P.A. Lespinat. 1985. Direct mass-spectrometric monitoring of the metabolism and isotope exchange in enzymic and microbiological investigations. In. Gas enzymology (Degn, H., et al., eds), pp. 17–35, Reidel Publishing Company, The Hague.
- Berlier, Y.M. and P.A. Lespinat. 1978 Relationship between nitrogenase and hydrogenase activity in a rhizosphese diazotroph. FEMS Microbiol. Lett. 3: 187–190.
- Dixon, R.O.D. 1972. Hydrogenase in legume root nodule bacteroids: Occurrence and properties. Arkh. Mikrobiol 85: 193–201.
- Dixon, R.O.D., Y.M. Berlier and P.A. Lespinat. 1981. Respiration and nitrogen fixation in nodulated roots of Soya Bean and Pea. Plant Soil 61: 135–143.
- Gow, L.A., I.P. Pankhania, S.P. Ballantine, D.H. Boxer and W.A. Hamilton. 1986. Identification of a membrane-bound hydrogenase ofDesulfovibrio vulgaris (Hildenborough). Biochim. Biophys. Acta 851, 57–64.
- Guarria, L.J. and H.D. Peck, Jr. 1971. Dinitrophenol-stimulated adenosine-triphosphate activity in extracts ofDesulfovibrio. J. Bacteriol. 106: 890–898.
- Hamilton, W.A. 1985. Sulphate-reducing bacteria and anaerobic corrosion. Annu. Rev. Microbiol. 39: 195–217.
- Hill, S., J.W. Drozd and J.R. Postgate. 1972. Environmental effects on the growth of nitrogen-fixing bacteria. J. Appl. Chem. Biotechnol. 22: 541–558.
- Keith, S.M. and R.A. Herbert. 1983 Dissimilatory nitrate reduction by a strain ofDesulfovibrio desulfuricans. FEMS Microbiol. Lett. 18: 55–59.
- Legall, J., D.V. DerVartanian and H.D. Peck, Jr. 1979. Flavoproteins, iron proteins and hemoproteins as electron transfer components of the sulfate-reducing bacteria. In: Current Topics in Bioenergetics (Sandy, R., ed.), pp. 237–265, Academic Press, New York.
- LeGall, J. and G. Fauque. 1987. Dissimilatory reduction of sulfur compounds. In: Biology of Anaerobic Microorganisms (Environmental Microbiolgy of Anaerobes) (Zehnder, A.J.B., ed.), John Wiley and Sons, New York, in the press.
- Le Gall, J. and J.C. Senez. 1960. Influence de la fixation de l'azote sur la croissance deDesulfovibrio desulfuricans. C.R. Acad. Sci. Paris 250: 404–406.
- Lespinat, P.A. and Y. Berlier. 1981. The dependence of hydrogen recycling upon nitrogenase activity inAzospirillum brasilense Sp7. FEMS Microbiol. Lett. 10: 127–132.
- Lissolo, T., S. Pulvin and D. Thomas. 1984. Reactivation of the hydrogenase fromDesulfovibrio gigas. J. Biol. Chem. 9: 11725–11729.
- Lissolo, T., E.S. Choi, J. LeGall, and H.D. Peck, Jr. 1986. The presence of multiple intrinsic membrane nickel-containing hydrogenases inDesulfovibrio vulgaris (Hildenborough). Biochem. Biophys. Res. Commun. 139: 701–708.
- Mortenson, L.E. 1978. Hydrogenase ofClostridium pasteurianum. In: Mechanisms of oxidizing enzymes (Singer, T., ed.), pp. 119–125. Elsevier, New York.
- Odom J.M. and H.D. Peck, Jr. 1981. Hydrogen cycling as a general mechanism for energy coupling in the sulfate-reducing bacteria. FEMS Microbiol. Lett. 12: 47–50.
- Peck, H.D., Jr. and J. LeGall. 1982. Biochemistry of dissimilatory sulfate reduction. In: Sulfur Bacteria (Postgate, J.R. and D.P. Kelly, eds.), pp. 448–466, The Royal Society, London.
- Postgate, J.R. 1984. The sulphate-reducing bacteria. Cambridge University Press.
- Smith, L.A., S. Hill and M.g. Yates. 1976. Inhibition by acetylene of conventional hydrogenase in nitrogen-fixing bacteria. Nature (Lond.) 262: 209–210.
- Van der Werf, A.N. and M.G. Yates. 1978. In: Hydrogenases: Their Catalytic Activity, Structure and Function (Schlegel, H.G. and K. Schneider, eds.), pp. 307–326. E. Goltz, Gottingen.
- Walker, C.C. and M.G. Yates. 1978. The hydrogen cycle in nitrogen-fixingAzotobacter chroococcum Biochimie 60: 224–231.
- The relationship between hydrogen metabolism, sulfate reduction and nitrogen fixation in sulfate reducers
Journal of Industrial Microbiology
Volume 1, Issue 6 , pp 383-388
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Sulfate-reducing bacteria
- Hydrogen metabolism
- Nitrogen fixation
- Deuterium-proton exchange
- Industry Sectors
- Author Affiliations
- 1. A.R.B.S.-Equipe Commune d'Enzymologie CNRS-CEA, C.E.N. Cadarache, 13108, Saint-Paul-lez-Durance, France
- 2. Department of Biochemistry, School of Chemical Sciences, University of Georgia, Boyd Graduate Studies Research Center, GA, USA