Journal of Industrial Microbiology

, Volume 1, Issue 3, pp 139–147

Purification and characterization of three proteins from a halophilic sulfate-reducing bacterium,Desulfovibrio salexigens

  • M. Czechowski
  • G. Fauque
  • N. Galliano
  • B. Dimon
  • I. Moura
  • J. J. G. Moura
  • A. V. Xavier
  • B. A. S. Barato
  • A. R. Lino
  • J. LeGall
Original Papers

DOI: 10.1007/BF01569265

Cite this article as:
Czechowski, M., Fauque, G., Galliano, N. et al. Journal of Industrial Microbiology (1986) 1: 139. doi:10.1007/BF01569265

Summary

Hydrogenase, desulfoviridin and molybdenum proteins have been isolated from a halophilic sulfate-reducing bacteria,Desulfovibrio salexigens strain British Guiana. At least 50% of the hydrogenase was found to be located in the periplasm. The hydrogenase has a typical absorption spectrum, a 400/280 nm ratio of 0.28, a molecular weight by sedimentation equilibrium of 81 000 and is composed of two subunits. It has one nickel, one selenium and 12 iron atoms per molecule. The sulfite reductase has a typical desulfoviridin absorption spectrum, a molecular weight of 191 000 and iron and zinc associated with it. The molybdenum-iron protein is gray-green in color and exhibits an absorbtion spectrum with peaks around 612, 410, 275 nm and a shoulder at 319 nm. It is composed of subunits of approximately 13 250 and has an approximate molecular weight of 110 000. Three molybdenum and 20 iron atoms are found associated with it.

An extensive study of these three proteins will allow a better understanding of the function of these enzymes and also of their possible role in microbially caused corrosion.

Key words

Halophilic sulfate-reducing bacteria D. salexigens Enzyme function Metal corrosion 

Copyright information

© Society for Industrial Microbiology 1986

Authors and Affiliations

  • M. Czechowski
    • 1
  • G. Fauque
    • 1
  • N. Galliano
    • 1
  • B. Dimon
    • 1
  • I. Moura
    • 2
  • J. J. G. Moura
    • 2
  • A. V. Xavier
    • 2
  • B. A. S. Barato
    • 2
  • A. R. Lino
    • 2
  • J. LeGall
    • 3
  1. 1.A.R.B.S.-Section Enzymologie Biochimie BactérienneCEN-CadaracheSaint Paul lez Durance CedexFrance
  2. 2.Centro de Química Estrutural, Complexo InterdisciplinarInstituto Superior TécnicoLisboaPortugal
  3. 3.Department of BiochemistryUniversity of Georgia, Boyd Graduate Studies Research CenterAthensUSA

Personalised recommendations