Current Microbiology

, Volume 5, Issue 2, pp 109–114

Polynucleotide sequence relatedness among motileAeromonas species

Authors

  • Michel Y. Popoff
    • Service des EntérobacteriesINSERM Unité 199, Institut Pasteur
  • Colette Coynault
    • Service des EntérobacteriesINSERM Unité 199, Institut Pasteur
  • Martine Kiredjian
    • Service des EntérobacteriesINSERM Unité 199, Institut Pasteur
  • Muguette Lemelin
    • Service des EntérobacteriesINSERM Unité 199, Institut Pasteur
Article

DOI: 10.1007/BF01567430

Cite this article as:
Popoff, M.Y., Coynault, C., Kiredjian, M. et al. Current Microbiology (1981) 5: 109. doi:10.1007/BF01567430

Abstract

Relatedness among 55 motileAeromonas strains was assessed by determining the extent of reassociation in heterologous DNA preparations. S1 nuclease and diethylaminoethyl-cellulose filters were used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of related nucleotide sequences. The motile aeromonads would presently consist of three species:A. hydrophila (type strain ATCC 7966),A. caviae (type strain ATCC 15468), andA. sobria (type strain CIP 7433). These three species are clearly separated on the basis of both biochemical characteristics and similarities in DNA. Each of these three species contains more than one hybridization group: three groups inA. hydrophila; two groups inA. caviae; and at least two groups inA. sobria. DNA hybridization groups are biochemically similar within each species. When additional data is available, these separate DNA hybridization groups may merit designation. Any decision to delineate species in terms of DNA hybridization groups must await a phenotypic basis for their separation.

Copyright information

© Springer-Verlag New York Inc 1981