Chromosome Research

, Volume 2, Issue 2, pp 99–113

cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association

Authors

  • K. Furukawa
    • Department of Biology, School of ScienceNagoya University
  • H. Inagaki
    • Department of Biology, School of ScienceNagoya University
  • T. Naruge
    • Department of Biology, School of ScienceNagoya University
  • S. Tabata
    • Department of Biology, School of ScienceNagoya University
  • T. Tomida
    • Department of Animal Science, School of Agricultural SciencesNagoya University
  • A. Yamaguchi
    • Laboratory of Reproductive BiologyNational Institute for Basic Biology
  • M. Yoshikuni
    • Laboratory of Reproductive BiologyNational Institute for Basic Biology
  • Y. Nagahama
    • Laboratory of Reproductive BiologyNational Institute for Basic Biology
  • Y. Hotta
    • Department of Biology, School of ScienceNagoya University
Research Papers

DOI: 10.1007/BF01553489

Cite this article as:
Furukawa, K., Inagaki, H., Naruge, T. et al. Chromosome Res (1994) 2: 99. doi:10.1007/BF01553489

Abstract

It is well known that cytoskeleton and karyoskeleton proteins are associated with changes in cell shape and with the rearrangement of the dynamic structures involved in cell division and motility. In higher vertebrates, there are three major skeletal protein groups: microfilaments, microtubules and intermediate filaments, each representing a multigene family. Some of these skeletal proteins are expressed in a temporally- and spatially-specific fashion, and they establish cell-specific cytoplasmic and nucleoplasmic organization during development. Here we report the cDNA cloning of a novel 60 kDa skeletal protein from mouse spermatocytes, termed MNS 1 (meiosis-specific nuclear structural protein), whose computer-predicted protein configuration indicates long α-helical coiled-coil domains flanked by non-helical terminal domains. Functional characterization of MNS1 by ectopic expression in culture cells indicated that it is a detergent-and high salt-resistant skeletal protein which is involved in organization of the nuclear or perinuclear architecture. The MNS1 protein is specifically expressed at the pachytene stage during spermatogenesis, so that its function may involve the determination and maintenance of the appropriate nuclear morphology during meiotic prophase.

Key words

cDNA cloningintermediate filamentmeiosismousenuclear behaviourskeletal proteinspermatogenesis

Copyright information

© Rapid Communications of Oxford Ltd 1994