Somatic Cell Genetics

, Volume 3, Issue 1, pp 47-60

First online:

Somatic cell genetic assignment of the human gene for mitochondrial NADP-linked isocitrate dehydrogenase to the long arm of chromosome 15

  • Nobuyoshi ShimizuAffiliated withDepartment of Biology, Yale University
  • , Richard E. GilesAffiliated withDepartment of Biology, Yale University
  • , Raju S. KucherlapatiAffiliated withDepartment of Biochemical Sciences, Moffett Laboratory, Princeton University
  • , Yoshiko ShimizuAffiliated withDepartment of Biology, Yale University
  • , Frank H. RuddleAffiliated withDepartment of Biology, Yale University

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A double-immunodiffusion method has been developed to detect human mitochondrial NADP-linked isocitrate dehydrogenase (EC; designated as IDH-2) using rabbit antiserum against the relevant enzyme. The method allows one to distinguish human IDH-2 from its mouse counterpart in extracts from human-mouse somatic cell hybrids. A correlation was found between the expression of human IDH-2 and the presence of human chromosome 15 in a “panel” of eight independent hybrid clones. Analysis of human marker enzymes for 37 different clones revealed a syntenic relationship between IDH-2 and mannose phosphate isomerase (EC; MPI), which has been assigned to chromosome 15 (1). These results permit the assignment of the structural gene for human IDH-2 to human chromosome 15. IDH-2 and human cytoplasmic IDH (IDH-1) were found to be asyntenic. Evidence from hybrid clones carrying a human X/15 translocation chromosome indicates that the human IDH-2 gene can be localized to the q11-qter region of chromosome 15.