Somatic cell genetic assignment of the human gene for mitochondrial NADP-linked isocitrate dehydrogenase to the long arm of chromosome 15
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- Shimizu, N., Giles, R.E., Kucherlapati, R.S. et al. Somat Cell Mol Genet (1977) 3: 47. doi:10.1007/BF01550986
A double-immunodiffusion method has been developed to detect human mitochondrial NADP-linked isocitrate dehydrogenase (EC 18.104.22.168; designated as IDH-2) using rabbit antiserum against the relevant enzyme. The method allows one to distinguish human IDH-2 from its mouse counterpart in extracts from human-mouse somatic cell hybrids. A correlation was found between the expression of human IDH-2 and the presence of human chromosome 15 in a “panel” of eight independent hybrid clones. Analysis of human marker enzymes for 37 different clones revealed a syntenic relationship between IDH-2 and mannose phosphate isomerase (EC 22.214.171.124; MPI), which has been assigned to chromosome 15 (1). These results permit the assignment of the structural gene for human IDH-2 to human chromosome 15. IDH-2 and human cytoplasmic IDH (IDH-1) were found to be asyntenic. Evidence from hybrid clones carrying a human X/15 translocation chromosome indicates that the human IDH-2 gene can be localized to the q11-qter region of chromosome 15.