, Volume 2, Issue 6, pp 537-544

Parameters of polyethylene glycol-induced cell fusion and hybridization in lymphoid cell lines

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Abstract

Methods employing polyethylene glycol (PEG) as a cell-fusion agent for mouse and human lymphoid cell lines were devised. Variables in these procedures were systematically explored. Under optimal conditions, PEG will induce 56% of the cells in the mouse cultures and 18% of the cells in the human cultures tested to undergo fusions resulting in multinucleated products. Intraspecific hybrids between mouse leukemic cells occur at a frequency of 26 × 10−5, and interspecific hybrids between mouse leukemic cells and Chinese hamster fibroblasts occur at 4 × 10−5. Differences in the requirements for maximum Sendai virus activity and PEG fusogenic activity are discussed.