Chromosome Research

, Volume 2, Issue 1, pp 65–71

Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley

  • Carsten Pedersen
  • Ib Linde-Laursen
Article

DOI: 10.1007/BF01539456

Cite this article as:
Pedersen, C. & Linde-Laursen, I. Chromosome Res (1994) 2: 65. doi:10.1007/BF01539456

Abstract

Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7I), 2 (2I), 4 (4I), and 5 (1I) by a two-stepin situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have disignated the new locusNor-16. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in otherTriticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

Key words

C-bandingchromosome identificationHordeum vulgarein situ hybridizationmicrosatellite

Copyright information

© Rapid Communications of Oxford Ltd 1994

Authors and Affiliations

  • Carsten Pedersen
    • 1
  • Ib Linde-Laursen
    • 1
  1. 1.Plant Biology Section, Environmental Science and Technology DepartmentRisø National LaboratoryRoskildeDenmark