Somatic Cell Genetics

, Volume 3, Issue 3, pp 303–312

Fusion of T and B cells


  • Georges Köhler
    • MRC Laboratory of Molecular Biology
  • Terry Pearson
    • MRC Laboratory of Molecular Biology
  • César Milstein
    • MRC Laboratory of Molecular Biology

DOI: 10.1007/BF01538748

Cite this article as:
Köhler, G., Pearson, T. & Milstein, C. Somat Cell Mol Genet (1977) 3: 303. doi:10.1007/BF01538748


Hybrid cells were prepared by fusing an immunoglobulinsecreting mouse myeloma line (B cell) with an allogeneic T-cell lymphoma which expresses the surface antigen Thy 1. The resulting hybrids expressed H2 antigens of both parental cells and secreted the immunoglobulin of the myeloma parent but did not express the Thy 1 antigen of the lymphoma parent. Twenty-one hybrids were formed from fusion of the same myeloma line with TNP-SRBC-primed spleen cells. Most of the hybrid lines exhibited characteristics expected for the fusion of the myeloma to B lymphocytes. No hybrids between the myeloma line and spleen T cells were identified as none of the hybrids expressed the T-cell-specific antigen Thy 1. We discuss possible reasons for failure to produce hybrids with T-cell characteristics in these types of fusion.

Copyright information

© Plenum Publishing Corporation 1977