Digestive Diseases and Sciences

, Volume 33, Issue 8, pp 1020–1024

Effects of graded α-glucosidase inhibition on sugar absorptionin vivo

  • H. Madariaga
  • P. C. Lee
  • L. A. Heitlinger
  • E. Lebenthal
Original Articles

DOI: 10.1007/BF01536000

Cite this article as:
Madariaga, H., Lee, P.C., Heitlinger, L.A. et al. Digest Dis Sci (1988) 33: 1020. doi:10.1007/BF01536000

Abstract

The effect of inhibition of disaccharidases on the degree of absorption of glucose, lactose, and sucrose was examined utilizing an in vivomodel in the rat. Acarbose, a competitive αglucosidase inhibitor was utilized to selectively inhibit small intestinal mucosal enzymes. Adult rats (250–350 g body weight) were the subjects of intraduodenal bolus infusion experiments with either sugar alone or sugar plus acarbose. All sugars were infused at a dose of 0.5 g/kg body weight. Portal venous blood glucose was determined at 30-min intervals from 0 to 150 min. Glucose (monosaccharide) and lactose (β-galactoside) absorption were not altered by the presence of acarbose. In contrast, sucrose (α-glucosidase) absorption was significantly diminished in the presence of acarbose. Sucrose absorption in the presence of increasing acarbose doses (0.7–5.6 mg/kg body weight) was depressed in a dose-dependent fashion. Linear regression analysis revealed a high degree of correlation between residual sucrase activity and area under blood glucose curve (r=0.9837). Similar degrees of correlation were found between acarbose dose and area under blood glucose curve (r=−0.9322), and between residual sucrase activity and acarbose dose (r=−0.9695). These data confirm that acarbose is a selective α-glucosidase inhibitor that does not affect monosaccharidase transport. In the presence of acarbose, α-glucosidase absorption is diminished in a dosedependent fashion. Postprandial glucose rise following an α-glucosidase meal seems to be determined, in the presence of graded acarbose inhibition, by residual mucosal α-glucosidase activity.

Key words

acarbose sucrase oligosaccharide digestion α-glucosidase inhibitors 

Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • H. Madariaga
    • 1
    • 2
  • P. C. Lee
    • 1
    • 2
  • L. A. Heitlinger
    • 1
    • 2
  • E. Lebenthal
    • 1
    • 2
  1. 1.International Institute for Infant Nutrition and Gastrointestinal DiseaseChildren's Hospital of BuffaloUSA
  2. 2.Department of Pediatrics, School of MedicineSUNY at BuffaloUSA
  3. 3.Division of GastroenterologyChildren's HospitalColumbus

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