Article

Somatic Cell and Molecular Genetics

, Volume 13, Issue 3, pp 235-244

First online:

Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line

  • Linda Cahilly-SnyderAffiliated withDepartment of Human Genetics, University of Pennsylvania, School of Medicine
  • , Teresa Yang-FengAffiliated withDepartment of Human Genetics, Yale University, School of Medicine
  • , Uta FranckeAffiliated withDepartment of Human Genetics, Yale University, School of Medicine
  • , Donna L. GeorgeAffiliated withDepartment of Human Genetics, University of Pennsylvania, School of MedicineHoward Hughes Medical Institute, University of Pennsylvania, School of Medicine

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Abstract

We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1and mdm-2.Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1–C3. Sequences homologous to mdm-1and mdm-2are present in the genomes of several species examined, including that of man.