Somatic Cell and Molecular Genetics

, Volume 13, Issue 3, pp 235–244

Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line

  • Linda Cahilly-Snyder
  • Teresa Yang-Feng
  • Uta Francke
  • Donna L. George
Article

DOI: 10.1007/BF01535205

Cite this article as:
Cahilly-Snyder, L., Yang-Feng, T., Francke, U. et al. Somat Cell Mol Genet (1987) 13: 235. doi:10.1007/BF01535205

Abstract

We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1and mdm-2.Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1–C3. Sequences homologous to mdm-1and mdm-2are present in the genomes of several species examined, including that of man.

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Linda Cahilly-Snyder
    • 1
  • Teresa Yang-Feng
    • 2
  • Uta Francke
    • 2
  • Donna L. George
    • 1
    • 3
  1. 1.Department of Human GeneticsUniversity of Pennsylvania, School of MedicinePhiladelphia
  2. 2.Department of Human GeneticsYale University, School of MedicineNew Haven
  3. 3.Howard Hughes Medical InstituteUniversity of Pennsylvania, School of MedicinePhiladelphia