Somatic Cell and Molecular Genetics

, Volume 15, Issue 6, pp 579–590

Chromosome localization and cDNA sequence of murine and human genes forras p21 GTPase activating protein (GAP)

  • C. -L. Hsieh
  • U. S. Vogel
  • R. A. F. Dixon
  • U. Francke
Article

DOI: 10.1007/BF01534919

Cite this article as:
Hsieh, C.L., Vogel, U.S., Dixon, R.A.F. et al. Somat Cell Mol Genet (1989) 15: 579. doi:10.1007/BF01534919

Abstract

The cDNA coding for mouse and human rasp21 GTPase-activating protein (GAP) was isolated; the deduced amino acid sequences share over 96% homology with that previously determined for bovine brain GAP. Both the mouse and human GAP cDNAs were used as probes for the chromosomal localization of this gene. The locus designations for the gene encoding GAP in human and mouse are RASAand Rasa (for ras-activating protein), respectively. By somatic cell hybrid analysis and in situ chromosomal hybridization, we have assigned the RASAgene to human chromosome band 5q13.3. In addition, with somatic cell genetics and linkage analysis in recombinant inbred mouse strains, the murine Rasagene was localized to the distal end of mouse chromosome 13. These assignments place the gene encoding GAP in a known conserved syntenic region.

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • C. -L. Hsieh
    • 1
  • U. S. Vogel
    • 2
  • R. A. F. Dixon
    • 2
  • U. Francke
    • 1
    • 3
  1. 1.Howard Hughes Medical InstituteStanford University Medical CenterStanford
  2. 2.Department of Molecular BiologyMerck Sharp and Dohme Research LaboratoriesWest Point
  3. 3.Departments of Genetics and PediatricsStanford University School of MedicineStanford