Somatic Cell and Molecular Genetics

, Volume 14, Issue 5, pp 415–426

Cloning and chromosomal location of human genes inducible by type I interferon

Authors

  • Marc G. Wathelet
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
  • Josiane Szpirer
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
  • Christine B. Nols
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
  • Isabelle M. Clauss
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
  • Lukas De Wit
    • Institut Pasteur du Brabant
  • M. Quamrul Islam
    • Department of GeneticsUniversity of Göteborg
  • Göran Levan
    • Department of GeneticsUniversity of Göteborg
  • Michel A. Horisberger
    • Pharmaceutical Research, Ciba-Geigy Ltd.
  • Jean Content
    • Institut Pasteur du Brabant
  • Claude Szpirer
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
  • Georges A. Huez
    • Département de Biologie MoléculaireUniversité Libre de Bruxelles
Article

DOI: 10.1007/BF01534709

Cite this article as:
Wathelet, M.G., Szpirer, J., Nols, C.B. et al. Somat Cell Mol Genet (1988) 14: 415. doi:10.1007/BF01534709

Abstract

When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-α, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2–5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2–5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.

Copyright information

© Plenum Publishing Corporation 1988