Somatic Cell and Molecular Genetics

, Volume 11, Issue 2, pp 117–126

Isolation and genetic characterization of human KB cell lines resistant to multiple drugs

  • Shin-ichi Akiyama
  • Antonio Fojo
  • John A. Hanover
  • Ira Pastan
  • Michael M. Gottesman
Article

DOI: 10.1007/BF01534700

Cite this article as:
Akiyama, S., Fojo, A., Hanover, J.A. et al. Somat Cell Mol Genet (1985) 11: 117. doi:10.1007/BF01534700

Abstract

Human KB cell lines resistant to high levels of colchicine were isolated by several successive single-step selections. Most of these selection steps resulted in cross-resistance to vincristine, vinblastine, adriamycin, actinomycin D, and puromycin; however, at the highest levels of colchicine resistance, increased cross-resistance to other drugs was not observed. There was no major change in protein synthesis or alteration in protein phosphorylation or [14C]glucosamine labeling patterns accompanying the development of multiple drug resistance as measured by analysis of metabolically labeled proteins on SDS gels. Cell-cell hybridization experiments showed that the colchicine-resistant and multiple drug-resistant phenotypes were incompletely dominant. In addition, colchicine resistance was found to segregate independently from resistance to other drugs in one somatic cell hybrid, suggesting that complex genetic loci are involved in the development of the multiple drug-resistant phenotype. These mutants should be useful for the study of the clinically important problem of multiple drug resistance in human cancer.

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Shin-ichi Akiyama
    • 1
  • Antonio Fojo
    • 1
  • John A. Hanover
    • 1
  • Ira Pastan
    • 1
  • Michael M. Gottesman
    • 1
  1. 1.Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis, National Cancer InstituteNational Institutes of HealthBethesda