Somatic Cell and Molecular Genetics

, Volume 11, Issue 3, pp 217–222

Assignment of gene coding human T-cell differentiation antigen, Tpl20, to chromosome 11

  • Ikuya Tsuge
  • Kazuhiko R. Utsumi
  • Ryuzo Ueda
  • Shigeru Takamoto
  • Toshitada Takahashi
Article

DOI: 10.1007/BF01534678

Cite this article as:
Tsuge, I., Utsumi, K.R., Ueda, R. et al. Somat Cell Mol Genet (1985) 11: 217. doi:10.1007/BF01534678

Abstract

A pan T-cell antigen with a molecular weight of 120 kilodaltons (kd) is recognized by a monoclonal antibody, Tp120, produced in our laboratories. Two hybrid clones reactive with this Tp120 antibody were established from the fusion between concanavalin A-stimulated human peripheral blood lymphocytes and hypoxanthine-guanine phosphoribosyltransferase-deficient mouse T cell leukemia, BW5147. These two clones were also positive with two other antibodies, 12.1 and T12, both of which detect 120kd pan T-cell antigen. Karyotype analysis showed that one clone retained human chromosomes 6, 7pq, and 11, and the other maintained chromosomes 11 and 21. As soon as both of these clones lost the chromosome 11, the expression of Tp120 became negative. The presence of human chromosome 11 was confirmed by the isozyme analysis of lactate dehydrogenase-A. The results indicated that the presence of chromosome 11 was essential for expression of 120kd pan T-cell antigen.

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Ikuya Tsuge
    • 1
  • Kazuhiko R. Utsumi
    • 2
  • Ryuzo Ueda
    • 3
  • Shigeru Takamoto
    • 3
  • Toshitada Takahashi
    • 2
  1. 1.Department of PediatricsNagoya University School of MedicineNagoya
  2. 2.Laboratories of Cell BiologyAichi Cancer Center Research InstituteNagoyaJapan
  3. 3.Laboratories of ChemotherapyAichi Cancer Center Research InstituteNagoyaJapan