Original Articles

Inflammation

, Volume 19, Issue 1, pp 133-142

First online:

Functional characterization of rat chemokine macrophage inflammatory protein-2

  • Charles W. FrevertAffiliated withPhysiology Program, Harvard School of Public Health
  • , Anthony FaroneAffiliated withPhysiology Program, Harvard School of Public Health
  • , Hadi DanaeeAffiliated withPhysiology Program, Harvard School of Public Health
  • , Joseph D. PaulauskisAffiliated withPhysiology Program, Harvard School of Public Health
  • , Lester KobzikAffiliated withPhysiology Program, Harvard School of Public HealthDepartment of Pathology, Brigham and Women's Hospital

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Abstract

Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50μg of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.