Cancer Immunology, Immunotherapy

, Volume 39, Issue 3, pp 161–166

Increased proliferation of a human breast carcinoma cell line by recombinant interleukin-2

Authors

  • Mitsuo Katano
    • Department of SurgerySaga Medical School
  • Tatsuya Matsuo
    • Department of SurgerySaga Medical School
  • Takashi Morisaki
    • Department of Ist SurgeryKyushu University School of Medicine
  • Keiko Naito
    • Department of Internal MedicineSaga Medical School
  • Fumio Nagumo
    • Hospital LaboratorySaga Medical School
  • Eiro Kubota
    • Department of Oral and Maxillofacial SurgerySaga Medical School
  • Mitsunari Nakamura
    • Department of SurgerySaga Medical School
  • Takeharu Hisatsugu
    • Department of SurgerySaga Medical School
  • Jutaro Tadano
    • Hospital LaboratorySaga Medical School
Original Articles Breast Carcinoma Cell Line, Interleukin-2, Interleukin-2 Receptor, Autocrine Growth Factor, Interleukin-2 Secretion

DOI: 10.1007/BF01533381

Cite this article as:
Katano, M., Matsuo, T., Morisaki, T. et al. Cancer Immunol Immunother (1994) 39: 161. doi:10.1007/BF01533381

Abstract

Two adenocarcinoma cell lines, Breast M25-SF and Breast M, were established from tumor tissue resected surgically from a patient with breast cancer. One, Breast M25-SF, expresses interleukin-2 receptor (IL-2R) on the cell surface and the other, Breast M, does not. The effects of recombinant inteleukin-2 (rIL-2) on the proliferation of these cell lines were investigated. The growth of Breast M25-SF was significantly promoted by rIL-2 ranging from 1,25 U/ml to 640 U/ml. Anti-CD25 (Tac) antibody, significantly blocked the growth enhancement of Breast M25-SF by rIL-2. Breast M, however, did not respond to rIL-2. To confirm more directly the promotion of Breast M25-SF growth by rIL-2, cloning of IL-2 responders from parent Breast M25-SF cells was carried out by limiting dilution without feeder cells in 96-well microplates. No colony formation was found in 24 wells without rIL-2. Eleven, 13 and 6 clones were established from groups of 24 wells containing rIL-2 at 200, 20 and 2 U/ml respectively. All of the clones expressed IL-2R and respond to rIL-2. By using a sensitive polymerase chain reaction technique, we demonstrated that Breast M25-SF but not Breast M expressed IL-2 mRNA, and IL-2 secretion from Breast M25-SF but not Breast M was also confirmed by radioimmunoassay. These findings suggest a role for IL-2 in autocrine support of Breast M25-SF growth. IL-2 may play an important role in the growth control of breast carcinoma cells.

Key words

Breast carcinoma cell lineInterleukin-2Interleukin-2 receptorAutocrine growth factorInterleukin-2 secretion

Copyright information

© Springer-Verlag 1994