Cancer Immunology, Immunotherapy

, Volume 39, Issue 3, pp 161–166

Increased proliferation of a human breast carcinoma cell line by recombinant interleukin-2

  • Mitsuo Katano
  • Tatsuya Matsuo
  • Takashi Morisaki
  • Keiko Naito
  • Fumio Nagumo
  • Eiro Kubota
  • Mitsunari Nakamura
  • Takeharu Hisatsugu
  • Jutaro Tadano
Original Articles Breast Carcinoma Cell Line, Interleukin-2, Interleukin-2 Receptor, Autocrine Growth Factor, Interleukin-2 Secretion

DOI: 10.1007/BF01533381

Cite this article as:
Katano, M., Matsuo, T., Morisaki, T. et al. Cancer Immunol Immunother (1994) 39: 161. doi:10.1007/BF01533381

Abstract

Two adenocarcinoma cell lines, Breast M25-SF and Breast M, were established from tumor tissue resected surgically from a patient with breast cancer. One, Breast M25-SF, expresses interleukin-2 receptor (IL-2R) on the cell surface and the other, Breast M, does not. The effects of recombinant inteleukin-2 (rIL-2) on the proliferation of these cell lines were investigated. The growth of Breast M25-SF was significantly promoted by rIL-2 ranging from 1,25 U/ml to 640 U/ml. Anti-CD25 (Tac) antibody, significantly blocked the growth enhancement of Breast M25-SF by rIL-2. Breast M, however, did not respond to rIL-2. To confirm more directly the promotion of Breast M25-SF growth by rIL-2, cloning of IL-2 responders from parent Breast M25-SF cells was carried out by limiting dilution without feeder cells in 96-well microplates. No colony formation was found in 24 wells without rIL-2. Eleven, 13 and 6 clones were established from groups of 24 wells containing rIL-2 at 200, 20 and 2 U/ml respectively. All of the clones expressed IL-2R and respond to rIL-2. By using a sensitive polymerase chain reaction technique, we demonstrated that Breast M25-SF but not Breast M expressed IL-2 mRNA, and IL-2 secretion from Breast M25-SF but not Breast M was also confirmed by radioimmunoassay. These findings suggest a role for IL-2 in autocrine support of Breast M25-SF growth. IL-2 may play an important role in the growth control of breast carcinoma cells.

Key words

Breast carcinoma cell lineInterleukin-2Interleukin-2 receptorAutocrine growth factorInterleukin-2 secretion

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Mitsuo Katano
    • 1
  • Tatsuya Matsuo
    • 1
  • Takashi Morisaki
    • 2
  • Keiko Naito
    • 3
  • Fumio Nagumo
    • 4
  • Eiro Kubota
    • 5
  • Mitsunari Nakamura
    • 1
  • Takeharu Hisatsugu
    • 1
  • Jutaro Tadano
    • 4
  1. 1.Department of SurgerySaga Medical SchoolSagaJapan
  2. 2.Department of Ist SurgeryKyushu University School of MedicineFukuokaJapan
  3. 3.Department of Internal MedicineSaga Medical SchoolSagaJapan
  4. 4.Hospital LaboratorySaga Medical SchoolSagaJapan
  5. 5.Department of Oral and Maxillofacial SurgerySaga Medical SchoolSagaJapan