Histochemistry and Cell Biology

, Volume 105, Issue 4, pp 253–260

Antigen retrieval, signal amplification and intensification in immunohistochemistry

Authors

  • Martin Werner
    • Institute of PathologyMedizinische Hochschule Hannover
  • Reinhard von Wasielewski
    • Institute of PathologyMedizinische Hochschule Hannover
  • Paul Komminoth
    • Division of Cell and Molecular Pathology, Department of PathologyUniversity of Zürich
Review

DOI: 10.1007/BF01463928

Cite this article as:
Werner, M., von Wasielewski, R. & Komminoth, P. Histochem Cell Biol (1996) 105: 253. doi:10.1007/BF01463928

Abstract

In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Moreover, we show the promising methods of signal amplification using biotinylated tyramine and signal intensification of diaminobenzidine reaction products by metallic ions.

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© Springer-Verlag 1996