European Archives of Oto-Rhino-Laryngology

, Volume 248, Issue 7, pp 406–408

A technique for quantitative cytology of nasal secretions

  • H. S. Lee
  • Y. Majima
  • Y. Sakakura
  • B. W. Kim
Original Investigations

DOI: 10.1007/BF01463564

Cite this article as:
Lee, H.S., Majima, Y., Sakakura, Y. et al. Eur Arch Otorhinolaryngol (1991) 248: 406. doi:10.1007/BF01463564

Summary

To quantitatively analyze cellular elements in mucopurulent nasal secretions from patients with chronic paranasal sinusitis, we studied five mucus-liquefying agents — dithiothreitol (DTT), ethylenediaminetetraacetic acid tetrasodium salt, propylene glycol, serratio peptidase and urea — with Hanks' balanced salt solution used as a control. Agents were compared for the number of cells, the mucus-liquefying effect and the staining characteristic of cells in cytocentrifuge-prepared smears. The results indicated that the treatment of nasal secretions with 10 mM DTT provides more clear and detailed cytological preparations for quantitative cytology of nasal secretions.

Key words

Quantitative cytologyNasal secretionsDithiothreitolChronic paranasal sinusitis

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • H. S. Lee
    • 1
  • Y. Majima
    • 1
  • Y. Sakakura
    • 1
  • B. W. Kim
    • 2
  1. 1.Department of OtorhinolaryngologyMie University School of MedicineTsu, MieJapan
  2. 2.Department of OtolaryngologyCatholic University Medical CollegeYongdungpo-ku, SeoulKorea