Genetica

, Volume 96, Issue 3, pp 269–283

The distribution of the transposable elementBari-1 in theDrosophila melanogaster andDrosophila simulans genomes

  • C. Caggese
  • S. Pimpinelli
  • P. Barsanti
  • R. Caizzi
Article

DOI: 10.1007/BF01439581

Cite this article as:
Caggese, C., Pimpinelli, S., Barsanti, P. et al. Genetica (1995) 96: 269. doi:10.1007/BF01439581

Abstract

The distribution of the transposable elementBari-1 inD. melanogaster andD. simulans was examined by Southern blot analysis and byin situ hybridization in a large number of strains of different geographical origins and established at different times.Bari-1 copies mostly homogeneous in size and physical map are detected in all strains tested. Both inD. melanogaster and inD. simulans a relatively high level of intraspecific insertion site polymorphism is detectable, suggesting that in both speciesBari-1 is or has been actively transposing. The main difference between the two sibling species is the presence of a large tadem array of the element in a well-defined heterochromatic location of theD. melanogaster genome, whereas such a cluster is absent inD. simulans. The presence ofBari-1 elements with apparently identical physical maps in allD. melanogaster andD. simulans strains examined suggests thatBari-1 is not a recent introduction in the genome of themelanogaster complex. Structural analysis reveals unusual features that distinguish it from other inverted repeat transposons, whereas many aspects are similar to the widely distributedTc1 element ofC. elegans.

Key words

Bari-1Drosophilagenomic organizationtransposons

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • C. Caggese
    • 1
  • S. Pimpinelli
    • 2
  • P. Barsanti
    • 1
  • R. Caizzi
    • 1
  1. 1.Instituto di GeneticaUniversità di BariBariItaly
  2. 2.Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia MolecolareUniversità di Roma ‘La Sapienza’RomaItaly