Protoplasma

, Volume 139, Issue 1, pp 20–24

Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum

  • R. Nobiling
  • H. -D. Reiss
Article

DOI: 10.1007/BF01417531

Cite this article as:
Nobiling, R. & Reiss, H.D. Protoplasma (1987) 139: 20. doi:10.1007/BF01417531

Summary

Pollen tubes ofLilium longiflorum were loaded with quin-2 to determine the cytoplasmic free calcium. Quin-2-fluorescence was detected at 500 nm with alternating excitation at 340 nm and 360 nm. The calcium2+-concentration was obtained using the intensity ratio R=I340/I360. The analysis exhibits a [Ca2+] of nearly 10−7mol·l−1 in the tip region and about 2·10−8 mol·l−1at the tube base, near the pollen grain. The data give evidence for the existence of a continuous gradient of free calcium within growing pollen tubes of various length.

Keywords

Calcium activityCalcium gradientLiliumPolar growthPollen tubeQuin-2Microfluorimeter

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • R. Nobiling
    • 2
  • H. -D. Reiss
    • 2
    • 1
  1. 1.ZellenlehreUniversität HeidelbergDeutschland
  2. 2.I. Physiologisches InstitutUniversität HeidelbergHeidelbergGermany