Acta Neurochirurgica

, Volume 133, Issue 1, pp 61–67

Transplantation of cultured sympathetic ganglionic neurons into Parkinsonian rat brain: Survival and function of graft

  • N. Nakao
  • T. Itakura
  • Y. Uematsu
  • N. Komai
Experimental Research

DOI: 10.1007/BF01404950

Cite this article as:
Nakao, N., Itakura, T., Uematsu, Y. et al. Acta neurochir (1995) 133: 61. doi:10.1007/BF01404950

Summary

The superior cervical ganglia (SCG) of newborn rats, which had been cultured as expiants for varying periods of time, were transplanted into the striatum of rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal dopamine pathway to examine the survival and functional properties of the sympathetic neurons maintained in long-term culture prior to grafting. In the rats given the SCG cultured in vitro for 2 weeks, apomorphine-induced rotational behaviour was satisfactory reduced. The rats receiving the SCG from 4-week-old cultures showed only modest behavioural changes. The grafting of the SCG cultured for 6 weeks in vitro did not affect the rotational behaviour. These behavioural data corresponded with the histological assessment of the graft survival by use of catecholamine histofluorescence. The present results suggest the critical time period in vitro which might allow the cultured sympathetic neurons to be successfully grafted.

Keywords

Parkinson's diseasesympathetic neuronstissue cultureneural transplantation

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • N. Nakao
    • 1
  • T. Itakura
    • 1
  • Y. Uematsu
    • 1
  • N. Komai
    • 1
  1. 1.Department of Neurological SurgeryWakayama Medical CollegeWakayama CityJapan