, Volume 174, Issue 1, pp 45–49

Azide treatment enhances cell-to-cell diffusion in staminal hairs ofSetcreasea purpurea

  • E. B. Tucker

DOI: 10.1007/BF01404041

Cite this article as:
Tucker, E.B. Protoplasma (1993) 174: 45. doi:10.1007/BF01404041


The effect of azide on the diffusion of fluorescent molecular probes was examined in staminal hairs ofSetcreasea purpurea. Staminal hairs were treated with azide before being microinjected with fluorescent molecular probes of different size, charge, and structure. The cell-to-cell movement of these fluorescent molecules was videotaped, analyzed, and coefficients of diffusion through plasmodesmata (D) and coefficients of diffusion across the tonoplast (k1) were calculated and compared to those of untreated cells. The D was larger and the k1 was smaller for many fluorescent probes in azide treated cells compared to normal, untreated cells. In addition, the cell-to-cell diffusion selectivity based on molecule structure, size and charge no longer existed in azide treated cells. An average D of 3.3×10−8cm2/s and an average k1 of 2.9×10−7/μm2/s was calculated for the molecular probes tested. New size limits for permeation were observed indicating that the plasmodesmata had become enlarged.


Cell-to-cell communication Plasmodesmata Setcreasea purpurea Azide 





diffusion coefficient for molecular probes in intercellular pores


fluorescein isothiocyanate-angiotensin II


coefficient of diffusive loss across the tonoplast

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • E. B. Tucker
    • 1
  1. 1.Department of Natural Siences, Baruch CollegeCity University of New YorkNew YorkUSA

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