Protoplasma

, Volume 162, Issue 1, pp 12–19

The effect of Cr (VI) on different inbred lines ofZea mays

I. Nuclei and cell cycle in the root tip tissue
  • M. G. Corradi
  • M. Levi
  • R. Musetti
  • M. A. Favali
Article

DOI: 10.1007/BF01403896

Cite this article as:
Corradi, M.G., Levi, M., Musetti, R. et al. Protoplasma (1991) 162: 12. doi:10.1007/BF01403896

Summary

Four inbred lines ofZea mays (33.16, B 68, N 7B, B 77) were grown in nutrient solution to which K2Cr2O7 was added to give final concentration of 5 mg/l Cr (VI). The most evident differences in metal tolerance were observed between the B 68 and 33.16 line: in fact, even though the level of Cr (VI) was almost the same in the root tissues of both lines after 6 d of treatment, in the B 68 line, Cr induced marked alterations of nuclear structure and a progressive arrest of the cell cycle in G 1. In the 33.16 line, on the contrary, the integrity of the nuclei was well preserved and the progression of the cell cycle was only barely affected.

Keywords

Zea maysChromiumRootsCell cycle

Abbreviations

Cr

(VI) hexavalent chromium

CRBC

chick red blood cells

DAPI

4′,6-diamidino-2-phenylindole

FCM

Flow cytometry

FM

Fluorescence microscopy

TEM

Transmission electron microscopy

Tris

10 mM Tris(hydroxymethyl)aminomethane

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • M. G. Corradi
    • 1
  • M. Levi
    • 2
  • R. Musetti
    • 1
  • M. A. Favali
    • 1
  1. 1.Institute of BotanyUniversity of ParmaParmaItaly
  2. 2.Department of BiologyUniversity of MilanoMilano