Planta

, Volume 169, Issue 1, pp 51–63

Selective expression of a probable amylase/protease inhibitor in barley aleurone cells: Comparison to the barley amylase/subtilisin inhibitor

  • J. Mundy
  • J. C. Rogers
Article

DOI: 10.1007/BF01369775

Cite this article as:
Mundy, J. & Rogers, J.C. Planta (1986) 169: 51. doi:10.1007/BF01369775

Abstract

We have cloned and sequenced a 650-nucleotide cDNA from barley (Hordeum vulgare L.) aleurone layers encoding a protein that is closely related to a known α-amylase inhibitor from Indian finger millet (Eleusine coracana Gaertn.), and that has homologies to certain plant trypsin inhibitors. mRNA for this probable amylase/protease inhibitor (PAPI) is expressed primarily in aleurone tissue during late development of the grain, as compared to that for the amylase/subtilisin inhibitor, which is expressed in endosperm during the peak of storage-protein synthesis. PAPI mRNA is present at high levels in aleurone tissue of desiccated, mature grain, and in incubated aleurone layers prepared from rehydrated mature seeds. Its expression in those layers is not affected by either abscisic acid or gibberellic acid, hormones that, respectively, increase and decrease the abundance of mRNA for the amylase/subtilisin inhibitor. PAPI mRNA is almost as abundant in gibberellic acid-treated aleurone layers as that for α-amylase, and PAPI protein is synthesized in that tissue at levels that are comparable to α-amylase. PAPI protein is secreted from aleurone layers into the incubation medium.

Key words

Abscisic acid and mRNA inductionAleuroneAmylase/Protease inhibitorGibberellin and mRNA inductionHordeum (aleurone protein)

Abbreviations

ABA

abscisic acid

ASI

barley amylase/subtilisin inhibitor

bp

nucleotide base pairs

Da

dalton

dpa

days post anthesis

GA3

gibberellic acid

PAPI

probable amylase/protease inhibitor

poly(A)RNA

polyadenylated RNA

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. Mundy
    • 1
  • J. C. Rogers
    • 2
    • 3
  1. 1.Department of BiotechnologyCarlsberg Research LaboratoryCopenhagenDenmark
  2. 2.Division of Hematology/Oncology, Department of Internal MedicineWashington University School of MedicineSt. LouisUSA
  3. 3.Department of BiologyWashington University School of MedicineSt. LouisUSA